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Brains were homogenized using lysis buffer for whole protein and mitochondrial protein extraction as previously described (Nguyen et al., Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes
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Mechanisms involved in the therapeutic effects of paeonia lactiflora pallas in rheumatoid arthritis
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HT affects approximately 12% of the general population and significantly impacts quality of life, as thyroid hormones regulate basal metabolic rate and influence multiple organ systems (Wroska et al., 2024)