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The hydrolysis of amides is catalysed by amidases, and peptidases

Cells were suspended in 300 l basic medium containing 14 mM glucose in HBSS with Ca 2+ and Mg 2+ , and removed the supernatant after centrifugation at 100 g for 30 sec

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For the digested samples, 200 L of pre-warmed (37C) digesta was added for 2 h and then removed, as its turbidity interfered with absorbance readings

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