At this stage of purification GST-X fusion should be clean and depending on the nature of furhter experiments that we want to perform, we can either elute the fusion with glutathione (excess of the glutathione will compete and displace GST-X protein from the beads) or cleave the GST tag and release protein X (see cartoon below)
Ookhtens M, Mittur AV, Erhart NA: Changes in plasma glutathione concentrations, turnover, and disposal in developing rats
While glutathione helps detoxify alcohol byproducts, drinking alcohol at the same time places additional stress on the liver
Glutathione is naturally occurring and generally considered safe when taken as directed under medical supervision
Arsenic metabolism in plants: an inside story
Tianzhen Chen, Shanghai Jiao Tong University, China Updates Copyright 2023 Watling, Jagasar, McCluskey, Warsh, Rhind, Truong, Chavez, Houle, Tong, Kish and Boileau