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It is noteworthy that patients with liver cirrhosis experience significant pathological changes in the abundance of gut microbiota, intestinal barrier function, BAs metabolism, and microbial metabolites

Moreover, recent studies have shown a strong relationship between bone metabolism and intestinal microbiota, and their potential effect on the risk of developing osteoporosis [10]

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The remaining pellets were resuspended in annexin V binding buffer 1 and 2.5 L of 50 g/mL propidium iodide (PI