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Biofilms were stained by removing the spent medium and filling the well with 2 ml of 1% crystal violet for 5 min, followed by three washing steps with deionized water

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Li J, Chen H, Wang B et al (2017) ZnO nanoparticles act as supportive therapy in DSS-induced ulcerative colitis in mice by maintaining gut homeostasis and activating Nrf2 signaling

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Since the body continuously produces melanin and faces ongoing oxidative stress, discontinuing glutathione supplementation typically results in a gradual return to baseline conditions