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The analysis steps were summarized as follows: (1) the extraction of total DNA from microbiota, (2) polymerase chain reaction (PCR) amplification, (3) purification and recovery, (4) fluorescence quantification, (5) sequencing library preparation, and (6) sequencing on the machine

Region-specific neuronal toxicity has been linked to metabolic reprogramming in astrocytes, particularly in response to chronic stress and disease pathology 74

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