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For histological and immunohistochemical quantifications, ANOVA followed by Bonferroni post hoc tests was used to control for type I error in multiple comparisons

Samples were analysed using high-resolution mass spectrometry (Q Exactive, Thermo Fisher) coupled with a Dionex Ultimate 3000 ultra-high-performance liquid chromatography (UHPLC) system (Thermo Fisher) as previously described with some modifications 37

HKLB2405)

CM, conditioned medium

This may provide a model why so many more females have symptoms from toxic environments than males

A major advantage of this model is that it can be controlled with a specific time and dose of Meth administration