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Rotate injection sites daily to prevent lipohypertrophy (localised fat accumulation caused by repeated insulin-like signaling at the same site)

On day 2 (test trial), NOR involved providing the mice 5 min to explore the same arena while either replacing one of the previous two items with a novel object of similar size but different shape (square), whereas OLT included relocating one of the previous two items to a different quadrant in the arena