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While the glycolytic pathway produces ATP and metabolic intermediates for biosynthesis, glutamine metabolism can supply amino acids, nucleic acids and glutathione necessary for cell proliferation

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In both types of cells, upon ER stress induction by thapsigargin (Tg) treatment, all the three UPR pathways were activated, as determined by phosphorylation of IRE1, PERK, eIF2, and cleavage of ATF6 to generate the activated form, as well as increased expression of the downstream transcription factors XBP1s and ATF4 (Fig