glutathione sepharose 4b resin Agarose | 4B-GLU carbon spacer arm GSTrap 4B 5 x 5

At the end of this step, 20 L of 200 mM DTT was added before digestion with 50 L trypsin overnight, at 37C

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Even though structural elements of the BBB are similar, there are molecular differences between animals and humans (O'Brown et al., 2018)

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doi: 10.1002/mrd.23524 28 LuQSMaLJiangWJWangXBLuM