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Mistake 3: Ignoring hydration

After FOXO4-DRI treatment, the staining of FOXO4 and p53-pS15 became weaker in the nucleus at 48 h and then disappeared with an accumulation in the cytoplasm at 72 h, especially in keloid fibroblasts

Its value is not an absolute constant but is highly sensitive to the purity of the sample, the precision of the measuring instrument, and the specific technique used during measurement

The acylated side chain and amino acid substitutions extend half-life in vivo and also improve storage stability

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