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65 ) (Addgene, 52961) for CRISPRCas9 KO or into pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro 66 (Addgene, 71236) for CRISPRi knockdown, as previously described 41

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3 Results 3.1 Characteristics of patients with AAV at diagnosis The median age of the study subjects was 63.0 (51.074.0) years, and 26 and 45 patients were males and females, respectively

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Based on the discussed results, antioxidants such as vitamins E and C, lipoic acid, and selenium have hypoglycemic effects, however, supplementation of patients with these vitamins did not bring the desired effect