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After removal and flushing with PBS, colon samples were cut into sections, pretreated to remove epithelial cells, then minced and digested with collagenase I, collagenase IV, and DNase to yield single-cell suspensions
Both ion envelopes are confirmed independently in the deconvoluted spectrum, so the pentadecapeptide and the 43-residue polypeptide are both validated against expected theoretical values before researchers who buy BPC-157 + TB-500 from Koi open the vial