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This guide explains the key differences between bacteriostatic water and sterile water, when each should be used, and why making the right choice is important

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Also, DAG might turn on an enzyme called protein kinase C (PKC), which may add phosphate groups to other signaling molecules

The advantage of on-column tag removal is that it eliminates the extra step of separating the cleaved protein from GST, since the GST-tag itself remains bound to the medium