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Eurasian Soil Sci

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(3) mention of prescribing whatever the banned substance may have been as treatment for the diagnosis

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After the percentage record of linear motile spermatozoa at 200 magnification under a phase contrast microscope in three different microscopic fields for each sample, the final motility ratio of frozen-thawed sperm motility in each group was calculated based on the mean of three successive estimations ( The frozen-thawed sperm viability was analyzed by eosin-nigrosin staining ( Giemsa staining of frozen-thawed spermatozoa (repeated in triplicated) was performed for the acrosome integrity assay ( After incubation with hypo-osmotic swelling solution [HOS, containing 6.75 g/L fructose and 3.67 g/L sodium citrate] at 37C for 1 h, plasma membrane integrity of each group (repeated in triplicated)was analyzed by evaluating the modifications of sperm tail to score swollen sperm according to previous publications ( For mitochondrial function assay, methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay was performed

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