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Reactive oxygen species (ROS) levels were assessed by C11-BODIPY probe, oxidative stress indicators including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase were measured by kits, and Cytochrome C, Ferritin, mitochondrial transcription factor A (TFAM), glutathione peroxidase 4 (GPX4) and SLC7A5 expression were detected by Western blot

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Samples were centrifuged at 4000 g for 25 min

Known to regulate gene expression and protein synthesis, epithalon has been found to suppress both the CCl11 and HMGB1 genes, thereby increasing the lifespan of test subjects

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