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The slides were then fixed with methanol/acetic acid (3:1), followed by denaturation with 2.5 M HCl

Metabolite analysis HCT116 ( PIK3CA -MT/WT) cells were cultured in 100 mm dishes with culture medium overnight

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PMID 23118745

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Simple evaporation of the volatile organic phase allows concentration of analytes, but matrix removal is limited, and the extent of phase separation needs to be evaluated carefully for reliable quantification