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Increasing levels of clusterization (2, 3 etc.) group proteins of increasing transcriptional coincidence, i.e., proteins whose transcript levels show coupled variations across the datasets

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After further culture of the cells they were resolved by sedimentation through 7% and 16% (w/v) iodixanol (800 g for 20 min

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Structural modifications allow them to stay in circulation for hours or even days: IGF-1 LR3 : 2030 hour half-life CJC-1295 with DAC : 58 day half-life PEG-MGF : detectable for up to 12 weeks Pharmacokinetic enhancements such as PEGylation or fatty acid acylation extend the peptides residence time, reduce renal clearance, and improve therapeutic utility. Veronese & Mero, BioDrugs What Determines How Long Peptides Stay in Your System

Becejac et al., 2018