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In parallel, IGF-1 may also promote expansion of Leydig cell mass and/or support their functional maturation, perhaps through modest mitogenic or differentiation-supporting pathways

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Lentiviruses encoding myc-tagged c-MET (myc-c-MET, WT, or mutant cloned into the pLVx-IRES-puro vector) were generated in HEK 293 T packaging cells following standard transfection protocol 43

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