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How It Works: A sample of blood is drawn from a vein and analyzed in a laboratory to measure vitamin B12 levels

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RNA isolation and quantitative RT-PCR gene expression levels The total RNA from the kidney tissue was isolated using the TRIzol reagent (Invitrogen) [27], and the cDNA was synthesized following the manufacturers protocol, using 1 g RNA (Prime ScriptTM RT reagent Kit, Takara)

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