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Subsequently, the cells were lysed in a hypertonic buffer comprising 20 mM Tris-HCl (pH 7.4), 300 mM NaCl, 10 mM MgCl 2 , 0.5% sodium deoxycholate, 1% Triton X-100, 1 mM DTT, 100 g/ml CHX, and 300 U/ml of RNase inhibitor

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