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Bridges RJ, Natale NR, Patel SA

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Due to its fundamental role in maintaining cellular homeostasis, glutathione remains one of the most widely studied endogenous molecules in redox biology and metabolic research

The agarose was then subjected to washing steps, including once with low salt buffer (20 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA), high salt buffer (20 mM Tris-HCl, pH 8.0, 0.5 M NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA), LiCl buffer, and twice with TE buffer

Additionally, nearest neighbour analysis was performed to assess mitochondrial network connectivity

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