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Western blot Cells were lysed on ice for 30 min in an ice-cold lysis buffer containing 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1% NP-40 (Carl Roth, #8040.3), and 5 mM EDTA, supplemented with PhosSTOP (Roche, #4906845001), cOmplete protease inhibitor cocktail (Sigma Aldrich, #11836153001), and 1 mM phenylmethylsulfonyl fluoride (PMSF

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