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Cells were collected by centrifugation at 5000 g at 4 C for 10 min

The short plasma half-life (~14 minutes) means peak levels are transient, which is why weekly or biweekly protocols are common in clinical practice

doi: 10.1093/jxb/erj196 51 MaternS.Peskan-BerghoeferT.GromesR.Vazquez KieselR.RauschT

Decreased phenol sulfotransferase activities associated with hyperserotonemia in autism spectrum disorders

The mechanism of enzyme regeneration is unclear, although we speculate that presence of a reducing agent such as dithiothreitol (DTT) in the assay buffer could facilitate the recovery

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