Additionally, the downstream effects on kidney organoid differentiation was measured by quantifying the expression of proteins such as SIX2, a marker for metanephric mesenchyme (MM) nephron progenitor cells (NPC) [22], and other characteristic kidney organoid proteins markers, like podocalyxin (PODXL), megalin (LRP2), and GATA-binding protein 3 (GATA3) on the final day of differentiation [8,9,10,11,12]
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By contrast, the expression of N-Flag-ICP27, which was tethered to the BoxB-containing PAS through its interactions with the BoxB sequences 39 , had no inhibitory effect (Fig
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