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Initial inhibitor screens were conducted in 200 l volumes in 96 well plates with 50 M inhibitor, 100 M NADPH, and 5 nM TGR in 0.1 M potassium phosphate (pH 7.4), 10 mM EDTA with a preincubation step of 15 min

The data were presented as the fold increase of the target gene expression, normalized to the housekeeping gene beta-actin, compared to unstimulated conditions

These mechanisms suggest that aescin may reduce neurodegenerative processes and maintain brain function

Glutathione nasal spray may interact with certain medications, such as chemotherapy drugs and immunosuppressants

The supernatants were used after filtration with a 0.2-m filter