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In addition, the Belgium group assessed the roles of Tyr 2 , Pro 5 , and Phe 6 in Ang IV by systematic incorporation of constrained residues

Before each experiment, cells were detached by trypsinization, counted using a hemocytometer, seeded in 24 well plates at 10,000 cells per well and incubated for 24 hrs prior to the initiation of each experiment

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