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Antibiotic efficacy is linked to bacterial cellular respiration

Samples were taken at 0, 2, 4, and 6 h after incubation

Cells were fixed immediately in 4% paraformaldehyde for 20 min at room temperature, washed thrice with PBS, incubated for 1 h in blocking buffer (3% BSA, 0.5% saponin in PBS) and probed overnight at 4 C with anti-cytochrome c antibody (BD Pharmigen 556432, 1:200 in blocking buffer)

The FDA's primary mandate is to protect public health by ensuring that all drugs, including compounded medications, are safe and effective for their intended uses