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Another significant benefit of B12 shots is their impact on mood and mental health

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To assess cell viability, 10 l CellTiter-Fluor (Promega) was added to black plates and incubated for 30 min in the dark before fluorescence was measured at 7-mm depth (gain 100)

To ensure that higher scores reflected a better outcome, the raw data for the appearance and symptom scales were scored as follows: not at all = 4, a little = 3, quite a bit = 2, and very much = 1

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We measured the withdrawal threshold at 2 and 6 h and 4 and 7 days after the incision