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the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1:5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

Milk immunoglobulins for health promotion

10.1016/j.jaip.2012.09.003 19

On the same note, the growth factor therapies are usually not very effective because they are quickly degraded, they show limited penetration into ischemic tissue and the diabetic cells do not respond to growth factor signalling in the most effective way