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No direct effect

All chromatography buffers were prepared in Milli-Q Ultrapure water (MilliporeSigma, Burlington, MA) with 1015 mM Tris-HCl (Thermo Fisher Scientific) and 0.005% Polysorbate 80 (PS80) (Croda, Snaith, United Kingdom), and the indicated sodium chloride (NaCl) (Teknova, Hollister, CA) concentration and pH were adjusted with either 50% sodium hydroxide (NaOH) (Thermo Fisher Scientific) or 37% hydrochloric acid (Thermo Fisher Scientific)

However, after 8 weeks of treatment with intranasal GHK-Cu, when mice were 22 months of age, both males and females showed an increase in alternation percentage

Biochemistry of oxidative stress

Mitochondria, which are structures within the cells of the RPE that act as energy powerhouses, are particularly susceptible to ROS-induced damage [17]