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A frozen and thawed vial should be discarded entirely, even if the solution appears normal after thawing

Cultured mammalian cells might offer a better option for producing properly folded and functional mammalian proteins with appropriate post-translational modifications (Geisse et al

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For the expression of GST-ARL8B, the plasmid was transformed into E.coli BL21 DE3 cells and induced with 0.5 mM IPTG overnight