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[DOI] [PMC free article] [PubMed] [Google Scholar] 111.Traynor A.M., Owens R.A., Coughlin C.M., Holton M.C., Jones G.W., Calera J.A., Doyle S

For the preparation of nuclear extracts, frozen liver pieces were homogenized in homogenization buffer (20 mM HEPES, 70 mM sucrose, 220 mM Mannitol, 2 mM EDTA, 0.5 mg/ml BSA, 0.1 mM PMSF, 1.0 mM DTT, pH 7.5)