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Copper peptides are structurally delicate

An alternative possibility involving S-2,4-dinitrophenylation of thiols or other cellular nucleophiles was excluded based on the lack of an equal effect of CDNB, which could produce a similar arylation without release of NO

L.LjubisavljevicM

For elution of the target protein, the interaction to the ligand is resolved by changing the buffer conditions, for example, the pH value, or by adding a specific competitor that supplants the target protein from the ligand resulting in dissociation of the target, while the ligand remains immobilized on the stationary phase

Lee WR, Pan TL, Wang PW, Zhuo RZ, Huang CM, Fang JY

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