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The Bradford assay was used to determine protein concentration of cell extracts, from there samples with the same amount of protein were separated using SDS-PAGE technology and transferred onto a PVDF membrane

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The combination of a BET inhibitor (+)-JQ-1 and a proteasome inhibitor bortezomib (BTZ) has been shown to decrease GPX4 expression, thereby inducing ferroptosis in TNBC [18]

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They found higher VDR expression among the suicide group compared to controls, and this up-regulation of VDR mRNA levels is partly attributed to vitamin D deficiency [39]