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B., & Jordt, S
The extracellular enzyme activities were determined by a 96-microplate fluorometric method, and polyphenol oxidase and peroxidase were determined by red purpurine colorimetry and pyrogallol colorimetry, respectively ( Glomalin-related soil protein (GRSP) determination: T-GRSP (total glomalin-related soil protein) and EE-GRSP (easily extracted glomalin-related soil protein) were extracted with sodium citrate solution and determined by Komas Brilliant Blue method ( Microbial biomass and community determination Microbial biomass carbon, nitrogen, and phosphorus were determined by the chloroform fumigation extraction method ( Data statistics and analysis For each variable that was measured, including tobaccos yield and quality, various extracellular enzyme activities, SOM, C, N, and P, and microbial biomass in PLFAs, the data were analyzed by one-way analysis of variance (ANOVA) using Fishers least significant difference (LSD) at P 0.05
