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Complex formation and gel filtration chromatography hTrxR1 mutant proteins were incubated with a 20-molar excess of hTrx1 mutant proteins in Tris buffer (50 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA, pH 7.6) containing 2.5 mM NADPH for 5 h at room temperature

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